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Effect Of Gonadotrophin Releasing Hormone (Gnrh) On Post Partum Ovarian Activity In Nili Ravi Buffaloes

By: Akhlaq Tabassam | Dr. Muhammad Aleem.
Contributor(s): Dr. Kazim Raza Chohan | Dr. Khalid | Faculty of Veterinary Sciences.
Material type: materialTypeLabelBookPublisher: 1995Subject(s): Department of TheriogenologyDDC classification: 0441,T Dissertation note: Sixteen pluriparus Nili-Ravi buffaloes at 30-35 days postpartum were used. These buffaloes were randomly divided into 2 groups i.e. viz A and B GnRH treated and control group (n=8). A group received single injection of 200jg GnRH and B group served as control. Blood sample were collected on the days 0 (first day of experiment) 4 and 8 of the experiment. At day 0, 4, 8, 12, 16 and 20 in control and treated groups the average size of follicles were 0.91±0.12 and 1.2±0.28 1.1±0.2 cm, 0.6±0.2 cm and 1.9±0.2, 0.37±0.17cm 1.87±0.3, 1.36±0.27, 1.4±0.24, 1.37±0.22, 1.35±0.24, 1.26±2.0 cm respectively. The average number of follicles in GnRH treated and control buffaloes were 1.5±0.17, 1.62±0.16, 1.5±0.21, 0.62±0.32, 1.5±0.3 and 0.37±0.17, 1.5±0.18, 1.5±0.18, 1.75±0.16, 1.36±0.15, 1.37±0.27, 1.25±0.18 at day 0, 4, 8, 12, 16 and 20 respectively. Two animal from treated group responded to GnRH and showed a rise in serum progesterone concentration 0.81, 3.40, 3.44 and .101, 2.93, 3.00 ng/ml at day 0, 4 and 8 respectively. One animal from control group showed a rise in P4 at day 4 only In GnRH group animal exhibited oestrus during 76.5±6.31 days of postpartum. In control group animal exhibited oestrus at 89.7±7.32 days of postpartum. From the present study it is concluded that GnRH treatment at 35 days postpartum can initiate cyclic ovarian activity, and GnRH can be used for luteinization of large follicle present on the ovary\ reduction in calving interval and postpartum interval to oestrus.
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Veterinary Science 0441,T (Browse shelf) Available 0441,T
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Sixteen pluriparus Nili-Ravi buffaloes at 30-35 days postpartum were used. These buffaloes were randomly divided into 2 groups i.e. viz A and B GnRH treated and control group (n=8). A group received single injection of 200jg GnRH and B group served as control. Blood sample were collected on the days 0 (first day of experiment) 4 and 8 of the experiment.

At day 0, 4, 8, 12, 16 and 20 in control and treated groups the average size of follicles were 0.91±0.12 and 1.2±0.28 1.1±0.2 cm, 0.6±0.2 cm and 1.9±0.2, 0.37±0.17cm 1.87±0.3, 1.36±0.27, 1.4±0.24, 1.37±0.22, 1.35±0.24, 1.26±2.0 cm respectively. The average number of follicles in GnRH treated and control buffaloes were 1.5±0.17, 1.62±0.16, 1.5±0.21, 0.62±0.32, 1.5±0.3 and 0.37±0.17, 1.5±0.18, 1.5±0.18, 1.75±0.16, 1.36±0.15, 1.37±0.27, 1.25±0.18 at day 0, 4, 8, 12, 16 and 20 respectively. Two animal from treated group responded to GnRH and showed a rise in serum progesterone concentration 0.81, 3.40, 3.44 and .101, 2.93, 3.00 ng/ml at day 0, 4 and 8 respectively. One animal from control group showed a rise in P4 at day 4 only

In GnRH group animal exhibited oestrus during 76.5±6.31 days of postpartum. In control group animal exhibited oestrus at 89.7±7.32 days of postpartum. From the present study it is concluded that GnRH treatment at 35 days postpartum can initiate cyclic ovarian activity, and GnRH can be used for luteinization of large follicle present on the ovary\ reduction in calving interval and postpartum interval to oestrus.

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